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The European Journal of Orthodontics 2007 29(4):404-407; doi:10.1093/ejo/cjm040
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© The Author 2007. Published by Oxford University Press on behalf of the European Orthodontics Society. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Oestrogenicity of orthodontic adhesive resins

T Eliades*, V Gioni**, D Kletsas**, AE Athanasiou* and G Eliades***

* Department of Orthodontics, School of Dentistry, Aristotle University of Thessaloniki
** Laboratory of Cell Proliferation and Ageing, National Centre for Scientific Research Demokritos, Athens
*** Department of Biomaterials, School of Dentistry, University of Athens, Greece

Address for correspondence Theodore Eliades, 57 Agnoston Hiroon Street, Nea Ionia, Athens 14231, Greece E-mail: teliades{at}ath.forthnet.gr


   Abstract

The purpose of this study was to assess the oestrogenic action of a chemically cured, no-mix (Rely-a-Bond) and a light-cured (Reliance) orthodontic adhesive resin. The adhesives were bonded to 40 stainless steel maxillary incisor brackets (Diamond) divided into two equal groups, employing a method which simulated the clinical handling of materials. In total, three series of specimens were prepared for each adhesive–bracket group. All specimens were immersed in normal saline. Samples of eluent were removed from each group at 1 day and 1 week following incubation and tested for oestrogenicity by measuring their effect on the proliferation of the oestrogen-responsive MCF-7 breast cancer cells, while an oestrogen-insensitive cell line (MB-231 human breast adenocarcinoma) was used as a control.

Three-way analysis of variance with adhesive, concentration of eluent, and immersion period were used as discriminating variables. No evidence was found of stimulation of proliferation of these cells, indicating the absence of any oestrogenicity of orthodontic adhesive eluents.


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