© 1996 by European Orthodontic Society
A molecular mechanism of integrin regulation from bone cells stimulated by orthodontic forces
*Department of Preventive Dental Science, University of Manitoba Canada
**Department of Orthodontics, University of Kiel Germany
***Departments of Anatomy and Oral Biology, University of Manitoba
****Faculty of Dentistry, University of British Columbia Canada
Address for correspondence R. S. Carvalho, Laboratory for Treatment for Skeletal Disorders, Department of Orthopedic Surgery, 11th Floor Enders Building, 320 Longwood Avenue, Boston, MA 02115, U.S.A.
The purpose of this paper is to discuss a molecular mechanism in the signal transduction pathways of the regulation of integrin genes taking place in bone cells as a result of orthodontic or mechanical stimulation. Human osteosarcoma (HOS) TE-85 cells were cultured in Dulbecco's modified Eagle's medium (DMEM)/F-12 and grown to confluency in Flexercell type I dishes and orthodontic forces were applied to the cells via an intermittent strain of 3 cycles/minute using the Flexercell Strain Unit System for periods of 15 and 30 minutes, 2 and 24 hours and 3 days. Antibodies against ß1 and 
1 integrins were immunolocalized in strained and unstrained cultures. Total RNA was extracted and cDNA probes were used to measure at various mRNA expression of ß1(1.2 kb) and v (1.1 kb) integrins. A cDNA probe for cyclophylin (750 b) was used for controls of gene expression. Results showed that mechanical stimulation caused a reorganization of integrin distribution in comparison with non-stimulated controls. mRNA for ß1 expression showed a marked increase at 30 minutes and 3 days, while mRNA levels for v did not change with strain. The selective expression of integrins mRNA is indicative of a specific gene regulation by mechanical stimulation in the cells studied.
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